Last edited by Yozshutilar
Wednesday, May 20, 2020 | History

3 edition of Protein-nucleic acid interaction found in the catalog.

Protein-nucleic acid interaction

Protein-nucleic acid interaction

  • 45 Want to read
  • 24 Currently reading

Published by Macmillan in Basingstoke .
Written in English

    Subjects:
  • Nucleic acids.,
  • Proteins.

  • Edition Notes

    Statementedited by Wolfram Saenger and Udo Heinemann.
    SeriesTopics in molecular and structural biology -- 10
    ContributionsSaenger, Wolfram., Heinemann, Udo.
    Classifications
    LC ClassificationsQP620
    The Physical Object
    Paginationx,209p.
    Number of Pages209
    ID Numbers
    Open LibraryOL14998746M
    ISBN 100333453212

    Find many great new & used options and get the best deals for Structural Studies of Protein-Nucleic Acid Interaction: The Sources of Sequence-Specific Binding by Thomas A. Steitz (, Paperback) at the best online prices at eBay! Free shipping for many products! The characterization of protein-nucleic acid interactions is necessary for the study of a wide variety of biological processes. One straightforward and widely used approach to this problem is the electrophoretic mobility shift assay (EMSA), in which the binding of a nucleic acid to one or more proteins changes its mobility through a nondenaturing gel by: 6.

    In molecular biology, quantitation of nucleic acids is commonly performed to determine the average concentrations of DNA or RNA present in a mixture, as well as their purity. Reactions that use nucleic acids often require particular amounts and purity for optimum performance. To date, there are two main approaches used by scientists to quantitate, or establish the concentration, of nucleic. Protein-nucleic acid interaction edited by Wolfram Saenger and Udo Heinemann, Macmillan Press, £ (x + pages) ISBN 0 2Author: Richard Calendar.

    Book Description. Since the publication of the first edition of Chemistry of Protein Conjugation and Cross-Linking in , new cross-linking reagents, notably multifunctional cross-linkers, have been developed and synthesized. The completion of the human genome project has opened a new area for studying nucleic acid and protein interactions using nucleic acid cross-linking reagents, and.   This protocol allows the detection of protein–nucleic acid interactions in plant cells and can be completed in Cited by:


Share this book
You might also like
Model Ts, Pep Chapels, and a Wolf at the Door

Model Ts, Pep Chapels, and a Wolf at the Door

North Dakota State Greats! (Carole Marsh North Dakota Books)

North Dakota State Greats! (Carole Marsh North Dakota Books)

Deacon Boggles struggle with a liver pad

Deacon Boggles struggle with a liver pad

Chiltons repair & tune-up guide, Toyota Celica/Supra, 1971-84

Chiltons repair & tune-up guide, Toyota Celica/Supra, 1971-84

Great Britain 1750-1950

Great Britain 1750-1950

Building a model for training SED adolescents to use and generalize verbal social skills

Building a model for training SED adolescents to use and generalize verbal social skills

The behavior of renal-regulating hormones during hypogravic stress

The behavior of renal-regulating hormones during hypogravic stress

The house weighs heavy

The house weighs heavy

Caste, status, group, aggregate, and class

Caste, status, group, aggregate, and class

Desalting and water treatment membrane manual

Desalting and water treatment membrane manual

Punk spunk

Punk spunk

Presidential address, July 1934

Presidential address, July 1934

theory of perception

theory of perception

Here lies

Here lies

Protein-nucleic acid interaction Download PDF EPUB FB2

In addition, the affinity and specificity of a particular protein–nucleic acid interaction can be increased through protein oligomerization or multi-protein complex formation (e.g., GCN4, glucocorticoid receptor, transcription initiation complexes, mRNA splicing complexes, RISC, etc.).

Protein–nucleic acid interactions are sensitive to the concentrations and nature of electrolyte ions in solution (Leirmo, Harrison, Cayley, Burgess, & Record, ).We evaluated the influence of ion composition on beacon binding to preformed Cas9/sgRNA(+ 89) complex and on the Cas9/sgRNA(+ 89) assembly in assay mixtures containing mM of either NaCl, KCl, or KGlu.

We studied the sequence-specific interaction of E. coli catabolite activator protein (CAP) with DNA as a model for protein-nucleic acid interactions [9]. The diffusion time of CAP-DNA ( μs) is comparable to that of free DNA ( μs), allowing sorting without detection-probability corrections.

The structural biology of protein-nucleic acid interactions is in some ways a mature field and in others in its infancy. High-resolution structures of protein-DNA complexes have been studied since the mid s and a vast array of such structures has now been determined, but surprising and novel structures still appear quite : $ Protein-nucleic acid binding specificity in major groove -specificity can be determined by interactions between bases and amino acid side chains -very specific reactions have to be in the exact right place, spacial resolution in the major groove.

ISBN: OCLC Number: Description: x, pages: Contents: DNA-protein interactions in the regulation of gene expression, Hippel and ; structures of protein-nucleic acid complexes in solution by electro-optical analysis, ke and ewicz; NMR studies of protein-DNA recognition - the interaction of LAC repressor headpiece with.

The book contains articles highlighting the rapidly evolving field of protein-nucleic acid interactions. Individual chapters demonstrate the diversity of biological systems to which protein-nucleic recognition is of central importance.

There are chapters on the interaction of small proteins with DNA and RNA as well as on chromatin organization. The monograph approaches the study of protein-nucleic acid interactions in two distinctive ways.

First, DNA-protein and RNA-protein interactions are presented together. Second, the first half of the book develops the principles of protein-nucleic acid recognition, whereas the second half applies these to more specialized : ISBN: OCLC Number: Description: x, pages: illustrations ; 24 cm.

Contents: DNA-protein interactions in the regulation of gene expression / Peter H. von Hippel and Otto G. Berg --Structures of protein-nucleic acid complexes in solution by electro-optical analysis / Dietmar Porschke and Jan Antosiewicz --NMR studies of protein-DNA recognition.

About this book. The structural biology of protein-nucleic acid interactions is in some ways a mature field and in others in its infancy. High-resolution structures of protein-DNA complexes have been studied since the mid s and a vast array of such structures has now been determined, but surprising and novel structures still appear quite frequently.

Hwang, H. & Myong, S. Protein induced fluorescence enhancement (PIFE) for probing protein-nucleic acid interactions. Chemical Society revi Cited by: 5.

Here, we review the state of the art in the discovery and development of protein-protein and protein-nucleic acid interaction inhibitors that block the main steps of the HIV-1 replication cycle, giving a medicinal chemistry-oriented view of strategies for inhibiting these regulatory interactions that are involved in the entry process, in the Cited by: Biomolecular Simulations: Recent Developments in Force Fields, Simulations of Enzyme Catalysis, Protein-Ligand, Protein-Protein, and Protein-Nucleic Acid Noncovalent Interactions Wei Wang, Oreola Donini, Carolina M.

Reyes, and Peter A. Kollman Annual Review of Biophysics and Biomolecular Structure PROTEIN-DNA RECOGNITIONCited by: from book The Principles of Nucleic Acid Structure (pp) Protein—Nucleic Acid Interactions. Protein-Nucleotide and Nucleic Acid Interaction and.

Nucleic acids are the biopolymers, or large biomolecules, essential to all known forms of term nucleic acid is the overall name for DNA and RNA. They are composed of nucleotides, which are the monomers made of three components: a 5-carbon sugar, a phosphate group and a nitrogenous the sugar is a compound ribose, the polymer is RNA (ribonucleic acid); if the sugar is derived.

The structural biology of protein-nucleic acid interactions is in some ways a mature field and in others in its infancy. High-resolution structures of protein-DNA complexes have been studied since the mid s and a vast array of such structures has now been determined, but surprising and novel structures still appear quite : Stephen Neidle.

Cite this entry as: () Protein-Nucleic Acid Interaction. In: Encyclopedia of Genetics, Genomics, Proteomics and Informatics. Springer, Dordrecht.

Nucleic Acids in Chemistry and Biology: protein-nucleic acid interactions and techniques used to analyse nucleic acids. In summary, this book provides an excellent overview of the chemistry and biology of nucleic acids, at a level that is suitable for use in university teaching, but with enough detail to be useful as a /5(6).

Protein–nucleic acid interactions play essential roles in various biological activities such as gene regulation, transcription, DNA repair and DNA packaging. Understanding the effects of amino acid substitutions on protein–nucleic acid binding affinities can help elucidate the molecular mechanism of protein–nucleic acid recognition.

Electrophoretic Mobility Shift Assay: Analyzing Protein – Nucleic Acid Interactions proteins that bind directly to the genomic DNA and those that only interact with other. The RNA template can leave the nucleus and carries with it the information to make protein, explain Drs. Mary Campbell and Shawn Farrell in their book "Biochemistry." Two additional types of RNA then interact with the template RNA to produce protein, building each strand one amino acid at a time, per instructions copied from DNA.

Structures of protein–nucleic acid complexes are extracted from the PDB as files in PDB format representing both PDB entries (asymmetric units) and biological units.

For each biological unit of X-ray files and for first models of nuclear magnetic resonance files, interactions between the protein and DNA or RNA are computed and by:   An overview of the biomolecules proteins and nucleic acids. This video covers their structure, function and purpose in biology.

Proteins are composed of linked amino acids, then folded into a.